Assessing polymorphism information content pic the canadian. This statistic is defined relatile to a particular type of pedigree. A comparison of polymorphism information content and mean of transformed kinships as criteria for selecting informative subsets of barley hordeum vulgare l. Assessing polymorphism information content pic using ssr. If possible give an easy example to manually calculate pic value. How to calculate polymorphic information content pic value for snp marker data manually. The program online marker efficiency calculator imec. New polymorphic microsatellite loci for theobroma cacao. Calculate heterozygosity h and polymorphism information content picfor variants with up to 20 alleles.
Online marker efficiency calculator botanical society of. This is especially useful for the selection of highly informative markers for use in family linkage studies. The polymorphism information content varied from 0. Botstein d, white rl, skolnick m, davis rw 1980 construction of a genetic linkage map in man using restriction fragment length polymorphisms. This tool allows determining polymorphic information content and heterozygosity values. Observed heterozygosity and the polymorphism information content pic values for each ssr marker were calculated using pic calculator. Perl scripts are freely available for download from. Perl scripts are freely available for download from usda. Informativeness can be quantitatively measured by a statistic called the polymorphism information content, or pic. How can i calculate the pic value during ssr microsatellite genotyping. Calculator of hardyweinberg equilibrium p,q chwe gene. Development and validation of new ssr markers from expressed.
This calculator uses the principle of hardyweinberg equilibrium to calculate heterozygosity h and polymorphism information content pic of up to five variants each with up to 20 alleles. The polymorphism information content of a marker is the probability that the marker genotype of the offspring of a heterozygous parent affected with a dominant disease allows one to deduce which. To accurately design plant genetic studies, the information content of utilized. The polymorphism information content pic values were computed using the. Analysis of allele fidelity, polymorphic information. Anova f statistic was used to detect the differences among the. Users can select two types of marker dominant or codominant. This calculator uses the principle of hardy weinberg equilibrium to calculate. How to calculate polymorphic information content pic. This calculator uses the principle of hardyweinberg equilibrium to calculate.
A comparison of polymorphism information content and mean. The polymorphic information content pic was calculated using the polymorphism information content calculator 43. The polymorphism information content pic value is commonly used in genetics as a measure of polymorphism for a marker locus used in linkage analysis. In this communication we have derived the uniformly minimum variance unbiased estimator of pic along with its exact variance. The data set is available for download, together with other example files. Molecular markers on local species of citrullus colocynthis. What is the difference between polymorphic information. Polymorphic information content pic and heterozygosity h. High quality example sentences with polymorphism information content in context from reliable sources ludwig is the linguistic search engine that helps you to write better in english. Polymorphism information content pic for each locus and locations were calculated using pic calc 0. An online program to calculate polymorphic information content for molecular genetic studies.
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